Q about BSA/FA's/Tween80

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Q about BSA/FA's/Tween80 - 2/21/2006 1:45:28 PM

goodBSA

Posts: 1
Status: offline

What would happen to BSA with endogenous fatty acids bound if it were in the presence of tween80? Inquiring minds want to know. Like would the tween80 displace the fatty acids under correct conditions? Thanks.

Post #: 1

RE: Q about BSA/FA's/Tween80 - 2/28/2006 11:10:04 AM

biotechnocrat

Posts: 2
Status: offline

I know each mole of BSA binds two moles of fatty acid, but I don't know the affinity for various fatty acids. It would seem that an excess of Tween would cause displacement - that is what happens when you add caprylic acid to stabilize BSA against heat.

(in reply to goodBSA)

Post #: 2

RE: Q about BSA/FA's/Tween80 - 2/28/2006 2:14:29 PM

immunoman

Posts: 3
Status: offline

I asked around and this is what I got from one chemist who works with blood fractionation:

Tween80 would not displace any fatty acids per se because fatty acids bind to albumin through their polar end, the carboxyl group. Tween80 would not have the conformational structure required to fit into any of
the fatty acid binding sites on BSA. Tween80 has no carboxyl group, it is a non-ionic surfactant but it could still interact with albumin by hydrogen bonding.

Hope this helps!

(in reply to biotechnocrat)

Post #: 3